Activity-Based Sensing

Leveraging Selective Chemistry to Decipher New Redox and One-Carbon Biology

We have pioneered the field of activity-based sensing, where we develop chemical sensors for biological analytes that achieve high selectivity using reaction chemistry rather than conventional lock-and-key binding approaches. By applying these chemical tools to enable real-time imaging of reactive oxygen species and one-carbon metabolites at the single- cell, tissue, and animal level, we elucidate principles of how these molecular signals influence fundamental biological processes spanning epigenetics to immune response.

Reviews

Activity-Based Sensing: A Synthetic Methods Approach for Selective Molecular Imaging and Beyond

207

Bruemmer, K. J.; Crossley, S. W. M.; Chang, C. J.

Angew. Chem. Int. Ed. 2020, 59, 13734–13762
Activity-Based Sensing Methods for Monitoring the Reactive Carbon Species Carbon Monoxide and Formaldehyde in Living Systems

193

Ohata, J.; Bruemmer, K. J.; Chang, C. J.

Acc. Chem. Res. 2019, 52, 2841-2848
Caged luciferins for bioluminescent activity-based sensing

190

Su, T. A.; Bruemmer, K. J.; Chang, C. J.

Curr. Opin. Biotechnology 2019, 60, 198-204
Inorganic Chemistry Approaches to Activity-Based Sensing: From Metal Sensors to Bioorthogonal Metal Chemistry

189

Iovan, D. A.; Jia, S.; Chang, C. J.

Inorg. Chem. 2019, 58, 13546-13560
Reaction-based small-molecule fluorescent probes for chemoselective bioimaging

103

Chan, J.; Dodani, S. C.; Chang, C. J.

Nature Chem. 2012, 4, 973-984
Boronate Oxidation as a Bioorthogonal Reaction Approach for Studying the Chemistry of Hydrogen Peroxide in Living Systems

83

Lippert, A. R.; Van de Bittner, G. C.; Chang, C. J.

Acc. Chem. Res. 2011, 44, 793-804

Reactive Oxygen Species

A tandem activity-based sensing and labeling strategy enables imaging of transcellular hydrogen peroxide signaling

218

Iwashita, H.; Castillo, E.; Messina, M. S.; Swanson, R.A.; Chang, C. J.

Proc. Natl. Acad. Sci. U.S.A. 2021, 118, e2018513118
Versatile Histochemical Approach to Detection of Hydrogen Peroxide in Cells and Tissues Based on Puromycin Staining

179

Chung, C. Y. S.; Timblin, G. A.; Saijo, K.; Chang, C. J.

J. Am. Chem. Soc. 2018, 140, 6109-6121
Nox2 redox signaling maintains essential cell populations in the brain

65

Dickinson, B. C., Peltier, J., Stone, D., Schaffer, D. V., Chang, C. J.

Nature Chem. Biol. 2011, 7, 106-112
Aquaporin-3 Mediates Hydrogen Peroxide Uptake to Regulate Downstream Intracellular Signaling

60

Miller, E. W.; Dickinson, B. C.; Chang, C. J.

Proc. Natl. Acad. Sci. USA 2010, 107, 15681-15686
A Selective, Cell-Permeable Optical Probe for Hydrogen Peroxide in Living Cells

26

Chang, M. C. Y.; Pralle, A.; Isacoff, E. Y.; Chang, C. J.

J. Am. Chem. Soc. 2004, 126, 15392-15393

Reactive Carbon Species

A Transfer Hydrogenation Approach to Activity-Based Sensing of Formate in Living Cells

250

Crossley, S. W. M., Tenney, L., Pham, V. N., Xie, X., Zhao, M. W., & Chang, C. J.

J Am Chem Soc. 2024, 146, 1-12
Ligand-Directed Approach to Activity-Based Sensing: Developing Palladacycle Fluorescent Probes That Enable Endogenous Carbon Monoxide Detection

209

Morstein, J.; Höfler, D.; Ueno, K.; Jurss, J. W.; Walvoord, R. R.; Bruemmer, K. J.; Rezgui, S. P.; Brewer, T. F.; Saitoe, M.; Michel, B. W.; Chang, C. J.

J. Am. Chem. Soc. 2020, 142, 15917–15930
Development of a General Aza-Cope Reaction Trigger Applied to Fluorescence Imaging of Formaldehyde in Living Cells

164

Bruemmer, K. J.; Walvoord, R. R.; Brewer, T. F.; Burgos-Barragan, G.; Wit, N.; Pontel, L. B.; Patel, K. J.; Chang, C. J.

J. Am. Chem. Soc. 2017, 139, 5338-5350
An Aza-Cope Reactivity-Based Fluorescent Probe for Imaging Formaldehyde in Living Cells

144

Brewer, T. F.; Chang, C. J.

J. Am. Chem. Soc. 2015, 37, 10886-10889
A Reaction-Based Fluorescent Probe for Selective Imaging of Carbon Monoxide in Living Cells Using a Palladium-Mediated Carbonylation

100

Michel, B. W.; Lippert, A. R.; Chang, C. J.

J. Am. Chem. Soc. 2012, 134, 15668-15671

Reactive Sulfur Species

Cell-trappable Fluorescent Probes for Endogenous Hydrogen Sulfide Signaling and Imaging H2O2-Dependent H2S Production

110

Lin, V. S.; Lippert, A. R.; Chang, C. J.

Proc. Natl. Acad. Sci. USA 2013, 110, 7131-7135
Reaction-Based Fluorescent Probes for Selective Imaging of Hydrogen Sulfide in Living Cells

79

Lippert, A. R.; New, E. J.; Chang, C. J.

J. Am. Chem. Soc. 2011, 133, 10078-10080

Activity-Based Sensing

Leveraging Selective Chemistry to Decipher New Redox and One-Carbon Biology

Related Publications

Reviews